“I really enjoyed the course, it was really challenging but I learned so much and can’t wait to use everything that I have learned.” Megan F., MilliporeSigma Recommend to anyone doing flow cytometry.” Svetlana I., IBBR/NIST/University of Maryland, CP In Addition to Immunophenotyping, Flow Cytometric Cell Counting and Nanoparticle Analysis will be Demonstrated.įlow Sorting of Peripheral Blood Mononuclear Cells (human) for Two Populations Special Emphasis on Sorting Conditions (parameters, factors, strategies) as well as the Specific Challenges Related to Cell Preparation, Efficiency, Recovery, and Alternate Techniques for Cell Population Identification. This Rotation Includes Automation Demonstrations Including Automated Compensation, Sample Labeling, Acquisition from a Multi-sampler and Analysis. Flow Cytometer Setup (MACSQuant three lasers) with Special Emphasis on Compensation of Complex Multi-color Panels and Troubleshooting Antibody Titrations as Preparative Tool for Panel Development Utilization of Instrument for Rare Cell Detection. Staining of Human PBMC with 7-color Immunophenotyping Flow Panel. Each attendee will setup a flow cytometer and perform manual compensation. Basics on Flow Cytometer Settings - Voltages, Gating, Dump Channels, Exclusion, and Compensation. Flow Cytometer Setup (BD Accuri two lasers) with Special Emphasis on Compensation. Staining of Human Peripheral Blood Mononuclear Cells (PBMC) (hands on for each participant) with Basic Flow Panel (4 colors). Preparation of Samples for Multicolor Analysis Flow Sorting Multicolor Analysis, Compensation and Flow Sorting Hands-on Flow Cytometry Exercises Using Miltenyi MACSQuant Analyzer Bio-Rad S3e flow sorter BD Accuri C6 Software training: Introduction to FlowJo Cytometry Analysis Software Introduction to Cyflogic Cytometry Analysis Software Flow Cytometry - Acquisition/Trouble Shooting/ Software Introduction to FCS Express Cytometry Analysis SoftwareĪttendees attend all lectures and labs listed. Basic Analytical Techniques, Principles, and Trouble Shooting Principles of Cell Staining, Choosing the Right Experimental Controls, Instrument Setup (baseline and compensation) Phenotyping and Analyzing Cell Populations by Flow Cytometry – How to present and interpret flow cytometric data Introduction to acquisition and analysis Software Purification and Labeling of Antibodies for Flow Cytometric and Other Applications Flow Sorting: Critical Experimental Issues and Instrument Setup Parameters Functional Flow Cytometric Assays
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